[ Problem Point ]
In order to collect samples after measurement, an analytical method that does not use additives was required.
[ ⇒Kaizen Point ]
Absorbance was measured at a UV wavelength of 280 nm to quantify protein.
For a crude protein solution containing various proteins, when the absorbance is 1 when measured using an optical cell with an optical path length of 1 cm, the protein concentration in the solution is approximately 1 mg/mL. Become.
By approximating the absorbance at 1 mg/mL protein concentration as 1 and measuring the absorbance at 280 nm, the protein concentration in the sample solution can be estimated.
The advantage of this method is that the protein concentration can be calculated simply by measuring the absorbance at 280 nm using an absorptiometer. Later samples can be collected.